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How to make/use tools for handling zebrafish

How to Make Embryo Loops

            Embryo loops are useful for orienting and pushing the embryos around gently for observation with a microscope.  These are constructed with small capillary tubes, fishing line, and super glue.  They are relatively easy to put together, and each person should make a couple of them in case replacements are needed.  There are four simple steps:

1. Cut a small piece of fishing line (about half an inch).

2. Bring the ends of the fishing line together to create a small loop.

3. Put the ends into one hole of the capillary tube, ensuring that the fishing line is still in its loop conformation.

4. Once the small loop of fishing line is inside the capillary tube, pour super glue into and around the capillary tube opening to guarantee that the loop remains inside the tube.